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Optimization in the recovery of a labile intracellular enzyme
Author(s) -
Augenstein D. C.,
Thrasher K.,
Sinskey A. J.,
Wang D. I. C.
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260161102
Subject(s) - homogenization (climate) , sonication , intracellular , enzyme , chemistry , biochemistry , kinetics , chromatography , biology , biodiversity , ecology , physics , quantum mechanics
A high molecular weight intracellular enzyme of Bacillus brevis ATCC 9999 is released when the organism is disrupted by sonication of homogenization. However, both processes also degrade the enzyme. Assays for protein release and specific enzymatic activity of the released protein indicate that both release and degradation can be represented by first‐order kinetic models. Utilization of the difference between the kinetics of release and degradation allows optimization in the recovery of this enzyme for both the sonication and homogenization processes.