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Studies on adsorption of amyloglucosidase on ion‐exchange resins
Author(s) -
Solomon B.,
Levin Y.
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160903
Subject(s) - cationic polymerization , succinylation , adsorption , chemistry , conjugate , maleic acid , copolymer , amylase , maleic anhydride , ethylene , organic chemistry , enzyme , polymer chemistry , catalysis , polymer , biochemistry , amino acid , lysine , mathematical analysis , mathematics
Highly charged water‐soluble conjugates of amyloglucosidase with a copolymer of ethylene‐maleic acid or styrene‐maleic acid were prepared and adsorbed on DEAE‐cellulose and other cationic resins. Negatively charged enzyme conjugates were also obtained by means of succinylation. The polyanionic conjugates of amyloglucosidase adsorbed on the cationic carriers show higher temperature stability than the native enzyme adsorbed on the same carriers under the same experimental conditions. The insoluble enzyme conjugate‐carrier complexes could be reused and retained their full activity while working continuously for three weeks. The application of the insoluble enzyme preparations with the above characteristics which could possibly satisfy the requirements for industry are discussed.