Premium
Preparation of immobilized lactase. Continued studies on the enzymatic hydrolysis of lactose
Author(s) -
Weetall H. H.,
Havewala N. B.,
Pitcher W. H.,
Detar C. C.,
Vann W. P.,
Yaverbaum S.
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160510
Subject(s) - lactase , lactose , hydrolysis , chemistry , enzyme , chromatography , biochemistry
A soluble fungal lactase (lactase‐W) of greater activity that the previously available fungal lactase (lactase‐M) has been covalently coupled to ZrO 2 ‐coated porous glass particles and 1 mm diameter porous TiO 2 particles. The immobilized lactase‐W appears to give results similar to the lactase‐M except for the operational half‐life. At 30°C the half‐life of the lactase‐M appears to exceed that of the lactase‐W by approximately 100 days under operational conditions.