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Continuous hydrocarbon fermentation with colloidal emulsion feed. A kinetic model for two‐liquid phase culture
Author(s) -
Yoshida Fumitake,
Yamane Tsuneo
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160507
Subject(s) - chemostat , emulsion , hexadecane , fermentation , aqueous two phase system , candida tropicalis , substrate (aquarium) , chromatography , aqueous solution , chemistry , chemical engineering , hydrocarbon , phase (matter) , yeast , organic chemistry , biochemistry , biology , bacteria , ecology , genetics , engineering
Candida tropicalis was cultured in a chemostat‐type fermentor with n ‐hexadecane, dispersed in water as submicron droplets, as the only carbon substrate. The emulsion as well as the aqueous medium were fed continuously into the fermentor. A Monod‐type equation can correlate the specific group rate in the continuous fermentor with the concentration of submicron droplets. The same equation can also be fitted to the data for the conventional‐type batch culture in the same fermentor in which an oil phase as well as an aqueous phase existed, if the hydrocarbon concentration in the aqueous phase excluding oil drops is employed as the substrate concentration. This demonstrates that Candida tropicalis takes up only submicron droplets of n ‐hexadecane as the carbon substrate.