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A generalized mathematical model for the growth kinetics of Saccharomyces cerevisiae with experimental determination of parameters
Author(s) -
Peringer P.,
Blachere H.,
Corrieu G.,
Lane A. G.
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160402
Subject(s) - michaelis–menten kinetics , kinetics , saccharomyces cerevisiae , chemistry , yeast , oxidative phosphorylation , bioenergetics , substrate (aquarium) , biomass (ecology) , biochemistry , thermodynamics , biology , enzyme , enzyme assay , physics , ecology , agronomy , quantum mechanics , mitochondrion
A general model of the kinetics of microbial growth has been developed involving the kinetics of incorporation of substrate into biomass and the maintenance energy requirements. Results obtained from batch cultures of the yeast Saccharomyces cerevisiae growing in synthetic media at pH 5.1 and 30°C permitted all biological parameters in the model to be calculated. Values obtained for these parameters were: maximum specific glucose uptake rate (μ S m ), 2.08 g/g biomass/hr; apparent Michaelis constant for glucose ( K S ), 0.1 g/liter (5.5 × 10 −4 M ) apparent Michaelis constant for oxygen ( K L ), 1.4% O 2 (3.2 × 10 −6 M ) quantitative index of the Pasteur effect ( b ), 4.9 × 10 −4 % −1 O 2 (207 M −1 ). Under conditions of strongly substrate‐repressed respiration the values obtained for Y ATP and P/O were constant over the course of the exponential phase of growth ( Y ATP = 10.4 g biomass/mole ATP; P/O = 3 moles ATP/atom 0). Mass balances for aerobic and anaerobic cultures confirmed the results obtained form the generalized model. Results presented suggested the operation of a mechanism for regulating energy‐yielding metabolism which involved an equilibrium between the systems of oxidative phosphorylation and dephosphorylation and was dependent upon the level of catbolite repression.