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The preparation of immobilized lactase and its use in the enzymatic hydrolysis of acid whey
Author(s) -
Weetall Howard H.,
Havewala Noshir B.,
Pitcher Wayne H.,
Detar Clarence C.,
Vann William P.,
Yaverbaum Sidney
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160302
Subject(s) - lactose , lactase , hydrolysis , chemistry , immobilized enzyme , chromatography , enzyme , whey protein , beta galactosidase , enzymatic hydrolysis , organic chemistry , biochemistry , escherichia coli , gene
The enzyme β‐galactosidase (lactose) obtained from several microbial sources was immobilized on zirconia‐coated porous glass particles. The immobilized enzymes were characterized by determining pH profiles, kinetic constants, thermal profiles, and operationalhalf‐lives in lactose and whey ultrafiltrate solutions. Studies were carried out on continuous reactor performance, and enzyme requirements for scale‐up were estimated. Lactose or whey hydrolyzed by this technique could find use commercially as a sweetener in a number of dairy products.