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Maintaining perpetual synchrony in HeLa S3 culture
Author(s) -
Thilly W. G.,
Nowak T. S.,
Wogan G. N.
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160111
Subject(s) - hela , suspension culture , thymidine , cell culture , chemistry , dna synthesis , dna , microbiology and biotechnology , biology , biochemistry , genetics
HeLa S3 cells in suspension culture are resynchronized in each successive generation by exposure to 0.25m M thymidine. The resynchronization approach appears to be superior to single‐ and double‐block techniques because the period of effective inhibition of DNA synthesis is significantly shortened. Gram quantities of highly synchronous viable cells have been produced on a daily basis.