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The concentration of protein solutions by normal freezing
Author(s) -
Janson JanChrister,
Ersson Bo,
Porath Jerker
Publication year - 1974
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260160104
Subject(s) - chemistry , crystallization , chromatography , protein crystallization , ionic bonding , ion , organic chemistry
An apparatus is described which is designed for preparative freeze concentration experiments by the technique of normal freezing. It has a capacity of approximately 22 liters distributed over twelve vessels. The influence of various geometrical and chemical parameters such as stirring speed, crystallization rate, and sample composition on the normal freezing of protein solutions are discussed. For dilute protein solutions (<0.1%) the concentration factor generally was 8‐ to 10‐fold with recoveries of 90–100 percent. With higher protein concentrations and at ionic strengths higher than approximately 0.05, the recovery was decreased. No loss of activity was detected when concentrating enzyme solutions.