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The effect of controlled feeding of glycerol on β‐galactosidase production by Escherichia coli in batch culture
Author(s) -
Gray Peter P.,
Dunnill Peter,
Lilly Malcolm D.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150614
Subject(s) - glycerol , escherichia coli , fed batch culture , food science , enzyme , chemistry , biochemistry , biology , carbon source , enterobacteriaceae , microbiology and biotechnology , fermentation , gene
A mutant of E. coli constitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low feeding rates resulted in breakdown of the β‐galactosidase formed and gave reduced final levels of the enzyme.