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An apparatus to measure the rate of oxygen evolution while maintaining pO 2 constant during photosynthetic growth in closed culture vessels capable of operation at increased hydrostatic pressures
Author(s) -
Pope D. H.,
Berger L. R.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150307
Subject(s) - photosynthesis , oxygen , algae , constant (computer programming) , hydrostatic equilibrium , oxygen evolution , hydrostatic pressure , limiting oxygen concentration , chemistry , clark electrode , atmosphere (unit) , analytical chemistry (journal) , thermodynamics , botany , environmental chemistry , biology , physics , biochemistry , electrode , electrochemistry , organic chemistry , quantum mechanics , computer science , electrolyte , programming language
A double polarographic device is described which permits regulation of dissolved oxygen concentration at any preset pO 2 in suspensions or cultures of photosynthetic algae. It also simultaneously permits measurements of the rate of oxygen evolution during photosynthesis. The apparatus is designed to operate in closed vessels in the absence of a gas phase, and may be used at increased pressures tested up to 500 atmospheres. Regulation of oxygen levels was maintained constant at various preset concentrations equivalent to atmospheric pO 2 values ranging between 0.02 and 0.21 at 25°C.