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Studies on immobilized papain
Author(s) -
Weetall H. H.,
Mason R. D.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150303
Subject(s) - papain , covalent bond , casein , porous glass , chemistry , amide , carboxylic acid , substrate (aquarium) , derivative (finance) , chromatography , organic chemistry , porosity , enzyme , biology , ecology , financial economics , economics
Papain was covalently coupled to ZrO 2− coated porous glass by several different methods. These derivatives were characterized and their operational half‐lives determined using casein substrate. Papain covalently coupled to the porous glass, previously converted to a carboxylic acid derivative, through amide linkage gave a 35 day operational half‐life.