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Fed‐batch hydrocarbon fermentation with colloidal emulsion feed
Author(s) -
Yoshida Fumitake,
Yamane Tsuneo,
Nakamoto KenIchiro
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150204
Subject(s) - emulsion , hexadecane , substrate (aquarium) , hydrocarbon , chemical engineering , yield (engineering) , colloid , fermentation , aqueous two phase system , candida tropicalis , aqueous solution , chemistry , phase (matter) , chromatography , carbon fibers , materials science , yeast , organic chemistry , biochemistry , biology , ecology , composite number , engineering , metallurgy , composite material
Candida tropicalis was cultured with n ‐hexadecane, dispersed in water as submicron droplets, as the only carbon substrate; the emulsion being fed continuously into a fermentor containing only an aqueous medium (fed‐batch culture). The results have demonstrated that the organism takes up hydrocarbon accommodated in the aqueous phase as submicron droplets. The cell/substrate yield for the linear growth phase, where growth was limited by the supply of the substrate, was much higher than the yield for the exponential growth phase.