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The influence of environmental conditions on the macromolecular composition of Candida utilis
Author(s) -
Alroy Y.,
Tannenbaum S. R.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150203
Subject(s) - dilution , nucleic acid , chemostat , rna , composition (language) , chemistry , food science , biochemistry , ribosomal rna , biology , protein biosynthesis , chromatography , bacteria , genetics , thermodynamics , physics , linguistics , philosophy , gene
Glucose‐limited chemostat cultures of Candida utilis were cultivated at various pH levels (3.0–7.5), temperatures (15–37.5°C), dilution rates (0.06–0.42 hr −1 ), and with different nitrogen sources (NH 4 +and NO 3 − ). The ratio of total nucleic acid to protein increased with increase in dilution rate at constant temperature and decreased with increase in temperature at constant dilution rate. The pattern of these variations is consistent with the hypothesis that the nucleic acid to protein ratio is a function of the ratio of the actual dilution rate to the critical dilution rate corresponding to each one of the cultivation temperatures. This ratio is called “reduced dilution rate.” A basis is proposed on which various microorganisms may be compared with respect to the ratios of cell protein to nucleic acid, RNA, ribosomal RNA, and polysomes.