Premium
Film and pore diffusion studies with immobilized glucose oxidase
Author(s) -
Rovito B. J.,
Kittrell J. R.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150111
Subject(s) - glucose oxidase , diffusion , immobilized enzyme , porous glass , porosity , chemistry , chemical engineering , particle (ecology) , chromatography , thiele modulus , materials science , packed bed , thermodynamics , organic chemistry , enzyme , mass transfer , physics , oceanography , engineering , geology
Quantitative studies are reported of external film and internal pore diffusion effects for glucose oxidase immobilized on porous glass in a continuous, tubular, packed bed reactor. The McCune and Wilhelm model for external film diffusion is shown to describe these data over a particle Reynolds number range from 0.2 to 25. The Thiele analysis provides a good description of internal pore diffusion resistances. It appears that immobilized enzymes free of internal diffusional restrictions generally require the use of supports with pore sizes larger than those currently available in porous glass.