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The kinetic and mass transfer behavior of immobilized invertase on ion‐exchange resin beads
Author(s) -
Kobayashi Takeshi,
MooYoung M.
Publication year - 1973
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260150105
Subject(s) - invertase , chemistry , pellets , diffusion , mass transfer , chromatography , substrate (aquarium) , ion exchange , hydrolysis , michaelis–menten kinetics , immobilized enzyme , kinetic energy , ion exchange resin , kinetics , analytical chemistry (journal) , ion , inorganic chemistry , enzyme , enzyme assay , organic chemistry , thermodynamics , materials science , composite material , physics , oceanography , quantum mechanics , geology
Immobilized invertase was prepared by binding native invertase to a polyamine type ion‐exchange resin. Kinetic behavior of the immobilized invertase on these small pellets was investigated in a packed‐bed reactor. For low flow rates, an effect of interparticle diffusion on the Machaelis constant was observed and a correlation was proposed to evaluate the effect. At high flow rates, Michaelis and inhibition constants were determined and compared with those for native invertase. With large pellets, intraparticle diffusion was found to be important and at high substrate concentrations, the effectiveness factor exceeded unity. Good agreement was found between the theoretical analyses and the experimental data.