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Utilization of organic nitrogen compounds by Hydrogenomonas eutropha
Author(s) -
Wixom Robert L.,
Sheng YungBoo,
Becker Richard S.
Publication year - 1972
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260140608
Subject(s) - chemistry , amino acid , amide , glutamine , organic chemistry , hydrolysis , transamination , glycine , tryptophan , nitrogen , stereochemistry , biochemistry
Abstract The chemolithotroph, Hydrogenomonas eutropha , was tested for its ability to utilize a variety of single nitrogen sources during growth in an atmosphere of H 2 O 2 CO 2 The present data show that H. eutropha can utilize the nitrogen from many, but not all, amino acids, several sulfur‐containing amino acids, glucosamine, and two aliphatic amides. The nitrogen concentration that supported maximum growth for NH 4 Cl, L ‐glutamate, L ‐glutamine, urea, and glycine was in the 0.010–0.019 M range. H. eutropha failed to remove the nitrogen from primary and secondary amines, eycloleucine, tert ‐ DL ‐leucine, DL ‐ p ‐fluorophenylalanine, DL ‐5‐methyltryptophan, creatine, and creatine. This microorganism was able to partially degrade at least six substituted indoles and/or tryptophan catabolites and six substituted imidazoles and/or histidine catabolites. All of a series of 17 dipeptides were able to serve as a nitrogen source for growth in the absence of NH 4 Cl. Extracts of H. eutropha were able to catalyze the hydrolysis of 16 α‐dipeptides, 2 tripetides, a tetrapeptide, a polypeptide, a β‐aspartyl peptide, 2 γ‐glutamyl peptides, a N‐acetyl amino acid, and 4 amino acid amides. These results emphasize the effectiveness of H. eutropha in utilizing a wide diversity of organic nitrogenous compounds containing amino and amide groups, heterocyclic rings, and peptide bonds.