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Pilot plant conversion of steroid using resting cell suspension as biocatalyst
Author(s) -
McGregor W. C.,
Tabenkin B.,
Jenkins E.,
Epps R.
Publication year - 1972
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260140512
Subject(s) - respiration , chemistry , antimycin a , substrate (aquarium) , aeration , oxygen , chromatography , cyanide , biotransformation , biochemistry , enzyme , botany , biology , inorganic chemistry , organic chemistry , ecology , electron transport chain
Resting cell suspensions of Sepedonium ampullosporum have been used successfully in the pilot plant, transformation of 9β 10α‐pregna‐4, 6‐dien‐3, 20‐dione to its 16α‐hydroxy derivative. The resting cell enzyme system acted as a stable respiratory unit up to 150 hr after resuspension in water. Semicontinuous addition of substrate to the same cell suspension reduced overall conversion lime by 67%. Aeration and agitation were important factors affecting conversion rates. The hydroxylating system had a critical oxygen concentration above 90% saturation of air in water. Hydroxylase activity was inhibited by cyanide an d totally inhibited by the respiratory inhibitor antimycin A at concentrations much less that that concentration required to block normal respiration.