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Preparation and characteristics of microcapsules containing asparaginase
Author(s) -
Mori Takao,
Sato Tadashi,
Matuo Yuhsi,
Tosa Tetsuya,
Chibata Ichiro
Publication year - 1972
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260140411
Subject(s) - polymerization , membrane , asparaginase , particle size , interfacial polymerization , chemistry , materials science , chemical engineering , chromatography , polymer , composite material , biochemistry , medicine , lymphoblastic leukemia , monomer , leukemia , engineering
Conditions for the preparation of microcapsules containing asparaginase by interfacial polymerization were investigated. The activity of microcapsules prepared under the optimal conditions was about 37% compared with that of native asparaginase. Particle size of microcapsules could be controlled by determining the stirring rate and concentration of Span 85. The membranes of microcapsules were resistant to mechanical shock or attack of chymotrypsin, and no leakage of asparaginase from microcapsules was observed.