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A pilot plant apparatus for continuous phased culture—some observations on oxygen usage by Candida utilis during the cell cycle
Author(s) -
Dawson P. S. S.
Publication year - 1971
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260130611
Subject(s) - oxygen , pilot plant , biology , botany , chemistry , chemical engineering , engineering , organic chemistry
Abstract An inexpensive scale‐up of the cyclone column apparatus ( Can. J. Microbiol. , 11 , 893 (1965)) is described which is suitable for pilot plant studies on the 10 liter scale. Details of its construction and operation are given. Experimental with phased cultures of C. utilis growing in the equipment are reported during which studies of dissolved oxygen and oxygen uptake were made over the cell cycle. Changes in both oxygen parameters occurred during the cycle and were useful as monitors of the growth. Cell of C. utilis were found to be very sensitive to changes in oxygen supply during the early stages of the cell cycle.