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Sequential 11α‐hydroxylation and 1‐dehydrogenation of 16α‐hydroxycortexolone
Author(s) -
Lee B. K.,
Brown W. E.,
Ryu D. Y.,
Thoma R. W.
Publication year - 1971
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260130405
Subject(s) - dehydrogenation , hydroxylation , chemistry , yield (engineering) , aspergillus ochraceus , substrate (aquarium) , acetone , organic chemistry , boron , stereochemistry , chromatography , catalysis , biology , enzyme , materials science , ecology , food science , mycotoxin , ochratoxin a , metallurgy
Two‐step microbial transformation of 16α‐hydroxycortexolone to its 1‐dehydro‐11α‐hydroxy derivative, without isolating an intermediate, was achieved with an overall yield of 72% of product at a steroid substrate concentration of 3 mg/ml. The process included formation of the cycloborate complex of the substrate, hydroxylation of the borate complex with a suspension of Aspergillus ochraceus mycelium in phosphate buffer, and dehydrogenation of the 11α‐hydroxylated intermediate with acetone‐dried Arthrobacter simplex cells. The desired product was then obtained by breaking the resultant borate complex through acidification.