Apparatus for continuous, large‐volume suspended cell cultivation | Zendy

Fontanges R. | Zendy; Deschaux P. | Zendy; Beaudry Y. | Zendy

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Apparatus for continuous, large‐volume suspended cell cultivation

Author(s) -

Fontanges R.,

Deschaux P.,

Beaudry Y.

Publication year - 1971

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260130402

Subject(s) - volume (thermodynamics) , chromatography , constant (computer programming) , contamination , environmental science , chemistry , pulp and paper industry , biology , computer science , ecology , thermodynamics , engineering , physics , programming language

A 30‐liter apparatus for growing cells continuously is described. Samples are taken automatically, thus obviating one of the main causes of contamination. In order to eliminate the continuous introduction of a gas stream, the pH was kept constant by means of the injection of NH 4 OH or NaOH. Under these circumstances, and by means of the two methods of agitation described—rotary and “by Vibromixer”—a quantity of cells of approximately 4 × 10 10 is harvested every 48 to 72 hr.

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