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Oxygen transfer and scale‐up in lysine production by Ustilago maydis mutant
Author(s) -
SanchezMarroquin A.,
Ledezma M.,
Barreiro J.
Publication year - 1971
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260130309
Subject(s) - fermentation , industrial fermentation , volume (thermodynamics) , sodium sulfite , chemistry , ustilago , sulfite , laminar flow , reynolds number , scale up , substrate (aquarium) , chromatography , analytical chemistry (journal) , thermodynamics , biochemistry , biology , physics , organic chemistry , ecology , classical mechanics , turbulence , sodium , gene
The oxygen transfer coefficient estimated by both sulfite and dynamic methods and some of the rheological properties of fermentation broths derived from the batch cultivation of a mutant of U. maydis in a sugar cane juice substrate, are used in a scaling‐up procedure on the basis of the power consumption per volume unit concept. The fluid was of the Binham plastic type; the Np ‐ N RE correlations showed that the modified Reynolds numbers of the flat‐blade turbine impellers were low, near to or in the laminar region; the Na ‐ Pg / P relations were established and then used in the calculation regardless of the geometrical dissimilarities of the vessels. A change of scale from 5 to 50 liters was calculated and operated keeping the power per volume value constant. Reproduction of lysine yields, 2.5–3.2 g/liter, was repeatedly reached in 8 successive runs.