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A comparative study of immobilized amyloglucosidase in a packed bed reactor and a continuous feed stirred tank reactor
Author(s) -
O'Neill S. P.,
Dunnill P.,
Lilly M. D.
Publication year - 1971
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260130302
Subject(s) - continuous stirred tank reactor , chemistry , packed bed , immobilized enzyme , maltose , plug flow reactor model , bioreactor , chromatography , batch reactor , amylase , continuous reactor , chemical engineering , substrate (aquarium) , catalysis , organic chemistry , enzyme , oceanography , engineering , geology
The catalytic activity of amyloglucosidase covalently attached to DEAE‐cellulose was studied in a packed bed reactor and a continuous feed stirred tank reactor (CSTR) for the reaction maltose → glucose. At low flow rates mass‐transfer limitations in the bed reactor lead to lower conversions for this reactor compared to the CSTR. Simple theoretical expressions for these reactors were compared with the experimental results. There are significant differences between the kinetic parameters and pH profile of the immobilized and free enzyme. The immobilized enzyme also showed greater stability at 50°C than did free amyloglucosidase. The temperature dependence of the reaction rate was the same for immobilized and free enzyme.