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Enzyme inactivation with shearing
Author(s) -
Charm S. E.,
Wong B. L.
Publication year - 1970
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260120615
Subject(s) - shearing (physics) , viscometer , shear rate , laminar flow , shear (geology) , capillary action , chemistry , shear flow , volumetric flow rate , chromatography , mechanics , materials science , rheology , composite material , physics , viscosity
Enzymes subjected to shearing in a viscometer are partially inactivated. It is possible with viscometry to calculate the degree of inactivation that occura when an enzyme solution flows through a capillary tube. When shear rate × exposure time is less than 10 4 , there is little or no inactivation. The masa average shear‐rate × time or shear, for laminar flow in a cylindrical tube is simply 16L/3D. It is surprising that for a single pass through a tube, the masa average shear is independent of flow rate and shear rate.