Enzyme inactivation with shearing | Zendy

Charm S. E. | Zendy; Wong B. L. | Zendy

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Enzyme inactivation with shearing

Author(s) -

Charm S. E.,

Wong B. L.

Publication year - 1970

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260120615

Subject(s) - shearing (physics) , viscometer , shear rate , laminar flow , shear (geology) , capillary action , chemistry , shear flow , volumetric flow rate , chromatography , mechanics , materials science , rheology , composite material , physics , viscosity

Enzymes subjected to shearing in a viscometer are partially inactivated. It is possible with viscometry to calculate the degree of inactivation that occura when an enzyme solution flows through a capillary tube. When shear rate × exposure time is less than 10 4 , there is little or no inactivation. The masa average shear‐rate × time or shear, for laminar flow in a cylindrical tube is simply 16L/3D. It is surprising that for a single pass through a tube, the masa average shear is independent of flow rate and shear rate.

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