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Dialysis fermentation. II. Kinetic analysis of salicylic acid production via cell growth and maintenance
Author(s) -
Abbott Bernard J.,
Gerhardt Philipp
Publication year - 1970
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260120408
Subject(s) - salicylic acid , fermentation , pseudomonas fluorescens , chemistry , dialysis , metabolism , biochemistry , food science , biology , medicine , bacteria , genetics
A kinetic analysis was made of the relationship between salicylate production from naphthalene and growth of Pseudomonas fluorescens in semicontinuous dialysis culture. The specific rates both of product formation and growth initially were increased by the diffusional withdrawal of salicylate, but subsequently were reduced to low levels despite continued salicylate removal. Productivity and growth were correlated by the Luedeking‐Piret equation in an initial nondialysis period and in the early stages of dialysis fermentation, when specific growth rates exceeded. 005 hr −1 . Below this level of growth at later stages of dialysis fermentation, the specific production rate was correlated only with total cell mass by a proportionality constant of .035 hr −1 , which was attributed to maintenance metabolism. Maintenance accounted for about 84% of the total salicylate produced, while growth‐associated metabolism accounted for the remainder.