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The relationship of oxidation‐reduction potential to the growth performance of tissue culture media poised prior to incubation
Author(s) -
Daniels W. F.,
Garcia L. H.,
Rosensteel J. F.
Publication year - 1970
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260120308
Subject(s) - laboratory flask , incubation , reduction potential , cell culture , incubation period , food science , centrifuge , growth medium , chemistry , biology , biochemistry , bacteria , inorganic chemistry , physics , nuclear physics , genetics
Earlier observations revealed that incubation of media and the attendant changes in oxidation‐reduction potential (ORP) were related to improved cell production. This is a report, of work done to show that the higher levels and increased rates of growth of cells grown in incubated medium are associated with the ORP level of the medium before inoculation of the medium with cells. Work was done using 250‐ml centrifuge spinner bottles as the culture vessels. Further work is needed to establish the desirability of deliberate poising of media prior to use for studies in small vessels and flasks.