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Experiments with lysis of living cells of Eremothecium ashbyii and of Methanomonas by microbial enzymes
Author(s) -
Carenberg C. O.,
Hedén C.G.
Publication year - 1970
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260120204
Subject(s) - lysis , microorganism , nucleic acid , lytic cycle , enzyme , yeast , cell wall , biochemistry , bacteria , organism , cell , extracellular , biology , microbiology and biotechnology , bacterial cell structure , chemistry , virus , paleontology , genetics , virology
The possibility to use microorganisms as human food is limited by several factors. The intact cell is resistant to digestion, the cell wall is unbalanced in essential amino acids, and the nucleic acids are said to be harmful. For using single cell protein as food it may thus be necessary to disrupt the cell wall and separate the protein from nucleic acid. This paper is concerned with the production and properties of extracellular enzymes able to lyse cell walls of microorganisms. Soil bacteria and actinomycetes have been cultivated and lytic enzymes from these organisms have been used to lyse living cells of the yeast like organism E. ashbyii . Efforts were also made to use these enzymes for lysing cell of a Methanomonas sp .