Premium
Continuous enzyme processing: The isolation of prolyl‐tRNA synthetase from Phaseolus Aureus
Author(s) -
Dunnill P.,
Currie J. A.,
Lilly M. D.
Publication year - 1970
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260120106
Subject(s) - phaseolus , extraction (chemistry) , centrifugation , enzyme , chromatography , chemistry , enzyme assay , biology , food science , biochemistry , botany
A pilot‐plant process has been developed for the continuous extraction and partial purification of prolyl‐tRNA synthetase from mung bean. The bean slurry was wet ground in a hammer mill, clarified by two‐stage centrifugation, and the protein in the effluent fractionated by precipitation at pH values of 5.2 and 4.2. The throughput was 13 kg dry bean/hr. The improved extraction process and reduced processing time resulted in an enzyme product with a specific activity 16 × that previously obtained in the batch process. The yield was also 50–60 times higher.