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Adriamycin, 14‐hydroxydaimomycin, a new antitumor antibiotic from S. Peucetius var. caesius
Author(s) -
Arcamone F.,
Cassinelli G.,
Fantini G.,
Grein A.,
Orezzi P.,
Pol C.,
Spalla C.
Publication year - 1969
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260110607
Subject(s) - mycelium , antibiotics , biology , chromatography , chemistry , botany , yeast extract , microbiology and biotechnology , biochemistry , fermentation
Streptomyces peucetius var. caesius , obtained from S. peucetius , the daunomycin producing microorganism, by mutagenic treatment, differs from the parent culture by the color of the vegetative and aerial mycelia and by its antibiotic, producing ability. S. peucetius var. caesius accumulates adriamycin in submerged and aerated culture on a medium containing glucose, brewer's yeast, and inorganic, salts both in shake flasks and in stirred fementers. Isolation of the product is performed by solvent extraction, chromatography on buffered cellulose columns, and crystallization as the hydrochloride. The new antitumor agent, adriamycin, is the 14‐hydroxv derivative of daunomyein.