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Changes in acid soluble nucleotide components of candida utilis during the cell cycle
Author(s) -
Hampton A. N.,
Dawson P. S. S.
Publication year - 1969
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260110605
Subject(s) - fractionation , metabolism , cell cycle , biochemistry , formate , chromatography , cell fractionation , chemistry , nucleotide , cell , biology , enzyme , gene , catalysis
Acid soluble extracts obtained at 30 min intervals from cells of C. utilis growing in synchrony in a phased culture (cycle time 51/2 hr) were fractionated on a Dowex‐1‐formate column. The series of fractionation profiles showed changes in number and amounts of components over the cell cycle. Transient accumulations of numerous components over the complex pool were observed. The significance of the changes are discussed in relation to practical applications and cell metabolism.