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Large scale production of transfer ribonucleic acids from E. coli K‐12 MO7
Author(s) -
Hancher C. W.,
Phares E. F.,
Novelli G. David,
Kelmers A. D.
Publication year - 1969
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260110603
Subject(s) - chromatography , chemistry , elution , ethanol precipitation , phenol , extraction (chemistry) , precipitation , transfer rna , phenol extraction , ethanol , cellulose , rna , biochemistry , organic chemistry , physics , meteorology , gene
An engineering‐scale procedure for the recovery of 300–400 g batches of mixed transfer ribonucleic acids is described. Semicontinuous growth of E. coli K‐12 MO7 yielded 77 kg of harvested cells in four days. Phenol extraction and ethanol precipitation recovered a crude tRNA material that was further purified by DKAE‐cellulose chromatography in runs of 1 × 10 6 A 260 units each on a 6 × 30 in. column using a 240 1, gradient elution. The purified tRNAs were partially concentrated and resolved into three groups.