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The conversion of benzyl penicillin to 6‐aminopenieillanie acid using an insoluble derivative of penieillin amidase
Author(s) -
Self D. A.,
Kay G.,
Lilly M. D.,
Dunnill P.
Publication year - 1969
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260110306
Subject(s) - penicillin amidase , chemistry , phenylacetic acid , cellulose , penicillin , amidase , chromatography , polyethylene glycol , yield (engineering) , ammonium , escherichia coli , derivative (finance) , streptomycin , nuclear chemistry , biochemistry , enzyme , organic chemistry , antibiotics , materials science , economics , financial economics , metallurgy , gene
Penicillin amidase was extracted from Escherichia coli ATCC 9637, grown on phenylacetic, acid and glutamate, and purified by fractional ion with streptomycin sulphate, ammonium sulphate and polyethylene glycol, followed by chromatography on DEAE–cellulose. The purification factor was 100–200 × and the overall yield was about 115%. The enzyme was chemically attached to derivatives of cellulose and the kinetics of these insolubilized penicillin amidase preparations was investigated.