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Effect of high substrate concentration and cell feedback on kinetic behavior of heterogeneous populations in completely mixed systems
Author(s) -
Ramanathan M.,
Gaudy A. F.
Publication year - 1969
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260110207
Subject(s) - dilution , substrate (aquarium) , chromatography , settling , flocculation , chemistry , bioreactor , kinetics , volumetric flow rate , chemical engineering , thermodynamics , environmental engineering , biology , environmental science , ecology , organic chemistry , physics , engineering , quantum mechanics
Growth kinetics of heterogeneous populations of sewage origin were studied in completely mixed reactors of the once‐through type at a high concentration of incoming substrate, 3000 mg/l glucose, and in systems employing cell feedback or sludge recycle at an incoming substrate concentration of 1000 mg/1 glucose. The recycle flow rate employed was 25% of the incoming feed flow, and the concentration of cells in the recycle was maintained as closely as possible at 150% of the cell concentration in the reactor. Studies were made at various dilution rates. Throughout these studies, batch experiments using cells grown at the various dilution rates were made to determine k s and μ m values. As in previous studios using heterogeneous populations, the relationship between specific growth rates μ and substrate concentration S was represented better by the Monod equation than by any other which was tested. The growth “constants” μ m , k s , and Y were found to fall in the same general range as those determined in previous studies in once‐through systems operated at 1000 mg/l glucose. It was observed that cell recycle, even at the relatively low concentration factor employed in these studies, greatly enhanced the flocculating and settling characteristics of the cells.