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Large‐Scale isolation of acid phosphatase from human erythrocytes
Author(s) -
Fisher R. A.,
Harris H.,
Houldsworth M.,
Lilly M. D.,
Dunnill P.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100609
Subject(s) - acid phosphatase , size exclusion chromatography , ammonium sulfate , ammonium sulfate precipitation , chemistry , enzyme , isolation (microbiology) , biochemistry , yield (engineering) , chromatography , phosphatase , adsorption , precipitation , phosphate , filtration (mathematics) , biology , microbiology and biotechnology , organic chemistry , materials science , mathematics , physics , statistics , meteorology , metallurgy
A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300‐fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 27%. Results for the type A enzyme are also presented.