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The cultivation of animal cells at controlled dissolved oxygen partial pressure
Author(s) -
Kilburn D. G.,
Webb F. C.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100607
Subject(s) - partial pressure , sparging , oxygen , chemistry , suspension (topology) , bioreactor , chromatography , suspension culture , cell culture , biology , organic chemistry , mathematics , homotopy , pure mathematics , genetics
A 3‐liter culture vessel has been developed for the growth of animal cells in suspension at controlled pH and dissolved oxygen partial pressure (pO 2 ). The culture technique allows metabolically produced CO 2 to be measured; provision can be made to control the dissolved CO 2 partial pressure. In cultures containing a low serum concentration, gas sparging to control pO 2 was found to cause cell damage. This could be prevented by increasing the serum concentration to 10%, or by adding 0.02% of the surface‐active polymer Pluronic F68. The growth of mouse LS cells in batch culture without pO 2 control was found to be limited by the availability of oxygen. Maximum viable cell populations were obtained when dissolved pO 2 was controlled at values within the range 40–100 mm Hg.