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Kinetics of gas diffusion in mammalian cell culture systems. II. Theory
Author(s) -
McLimans William F.,
Blumenson Leslie E.,
Tunnah Kenneth V.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100604
Subject(s) - diffusion , oxygen , kinetics , tissue culture , solubility , chemistry , isolation (microbiology) , cell culture , thermodynamics , biophysics , biology , biochemistry , in vitro , microbiology and biotechnology , physics , organic chemistry , genetics , quantum mechanics
Mathematical models have been constructed which relate the depth of the culture fluid overlay to the oxygen available to mammalian cells cultured under static conditions. These models suggest that the maintenance of a given rate of oxygen utilization by some culture systems may be critically depended on this fluid depth and on the solubility and rate of diffusion of oxygen in the culture fluid. The importance of these concepts as applied to the isolation and growth of differentiated cells representative of the tissue of origin are noted.