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Emetic material associated with Fusarium species in cereal grains and artificial media
Author(s) -
Prentice Neville,
Dickson A. D.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100403
Subject(s) - fusarium , inoculation , poaceae , biology , cellulose , botany , chemistry , horticulture , biochemistry
Abstract Substances which cause emesis in pigeons were extracted from corn ( Zea mays ) artificially inoculated with Fusarium graminearum and from liquid culture medium inoculated with F. moniliforme, F. roseum, F. poae, F. culmorum , and F. nivale . Emetic preparations were obtained also from infected wheat ( Triticum aestivum L. em. Thell), ( Hordeum vulgare L. em. Lam), and durum ( Triticum durum Desf). Partial purification resulted from chromatography with columns of cellulose and DEAE cellulose and with thin layers of silica gel. Two active materials were obtained from liquid culture of F. moniliforme but only one from infected cereals. Emetic preparations from F. moniliforme and infected cereals contained a polypeptide as a minor component. Ultraviolet and infrared spectrums, elemental analyses, refractive indices, and amino acid composition of the emetic from corn and one of the emeties from liquid culture of F. moniliforme were similar but not identical. Attempts to crystalline these emetics and to characterize them were unsuccessful.