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Transient response of continuous cultures to changes in temperature
Author(s) -
Ryu D. Y.,
Mateles R. I.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100311
Subject(s) - transient (computer programming) , chemostat , steady state (chemistry) , arrhenius equation , growth rate , transient response , limiting , chemistry , thermodynamics , biology , physics , mathematics , activation energy , mechanical engineering , genetics , geometry , bacteria , computer science , electrical engineering , engineering , operating system
The purpose of this experimental study was to examine the transient response of a chemostat‐type continuous culture of Escherichia coli B to step changes in temperature by following transient limiting substrate concentration and calculating from it the transient growth rate. The transient response to step changes of temperature was tested for four different situations. In the first two cases, temperature was shifted down from 37 to 27°C., and 37 to 32°C. In the last two, it was shifted up from 32 to 37°C., and 27 to 37°C. When the temperature was shifted up, the growth rate increased rather rapidly to its transient maximum value and then decreased slowly until it, settled back into the steady‐state value. On the other hand, when the temperature was shifted down, the growth rate decreased relatively rapidly to its transient minimum and then it slowly increased and returned gradually to the steady‐state value. The magnitude of the transients was less than would be expected if the transient growth rates followed an Arrhenius function.