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Continuous flow cultures of a HeLa cell line as a basis for a steady supply of Rubella virus
Author(s) -
Holmström Björn
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100310
Subject(s) - hela , dilution , suspension culture , cell culture , volume (thermodynamics) , continuous flow , chromatography , virus , liter , biology , chemistry , virology , microbiology and biotechnology , genetics , physics , endocrinology , quantum mechanics , mechanics , thermodynamics
A HeLa cell line was propagated in semicontinuous suspension culture, 85 liters final volume, and in continuous flow culture with a volume of 300 ml. or 5 liters in an autoclavable medium to which 8% calf serum had been added. A medium containing 0.1% Methocel and 2% calf serum was also tested. Maximum productivity was obtained at a dilution rate of 0.33 day −1 with a cell density of about 1.0 × 10 6 cells/ml. The same cell line was also infected with Rubella virus and the production of virus was followed at the 5‐liter cultivation level.