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The preparation of rat‐liver mitochondria by conventional differential centrifugation and by zonal Centrifugation, after various disruption procedures: A comparative study
Author(s) -
Klucis E.,
Lloyd D.,
Roach G. I.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100307
Subject(s) - centrifugation , differential centrifugation , mitochondrion , homogenization (climate) , chromatography , polarography , chemistry , biochemistry , biology , biodiversity , ecology , inorganic chemistry
Rat liver was subjected to three different, disruption procedures (homogenization, explosive decompression, and Chaikoff press) and mitochondria were subsequently isolated by conventional differential Centrifugation and by zonal Centrifugation. The properties of these mitochondria were investigated by polarographic measurement of oxygen uptake and they were examined by electron microscopy. All three methods of disruption gave mitochondria which showed respiratory control. Nitrogen cavitation gave the most reproducible conditions for cell breakage and zonal Centrifugation gave good separation of subcellular organdies in extracts produced by this method. Some separation of the heterogenous mitochondrial populations was achieved by zonal Centrifugation.