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Apparatus for simultaneous spectrophotometric and polarographic measurements
Author(s) -
Ribbons D. W.,
Hewett A. J. W.,
Smith F. A.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100210
Subject(s) - quartz , electrode , polarography , analytical chemistry (journal) , range (aeronautics) , situated , materials science , chemistry , computer science , composite material , inorganic chemistry , chromatography , artificial intelligence
We have recently constructed a 10‐mm, light path quartz cuvet which will accept a Clark oxygen electrode; it is temperature controlled and is suitable for use in a Unicam (Cambridge, England) SP 800 recording spectrophotometer. Several enquiries have prompted this publication, although such an apparatus was mentioned much earlier by Chance and Williams, 1 and has been used extensively. Figure 1 a , b , c , and d and their legends provide sufficient detail for the construction of the cuvet and provision of the commercially available electrode, quartz faces, stirring motor and disk magnet. Circuit diagrams for temperature control (range 22–38°C., ± 0.2°C.) and stirrer speed control are shown in Figure 2 a and b . The cuvet is shown situated in the spectrophotometer cell housing in Figure 3, and the cuvet with its associated equipment is shown in Figure 4.