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Studies with dried airborne vaccinia virus in a small environmental chamber
Author(s) -
Orlando M. D.,
Riley J. M.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100204
Subject(s) - diluent , vaccinia , relative humidity , lactose , chemistry , virus , raffinose , chromatography , food science , virology , biology , biochemistry , sucrose , nuclear chemistry , physics , gene , recombinant dna , thermodynamics
Dried vaccinia virus was found to be very stable in aerosol form in our chamber at a temperature of 75°F and a relative humidity (RH) of 85% when the virus was protected with 1.5% lysine, 1.5% sodium glutamate, 0.5% isoniazid, 0.5% thiourea, and either 2.5% heart infusion broth (HIB) or a combination of 3.75% lactose plus 1.25% raffinose prior to freeze‐drying. Utilization of the Environmental chamber technique resulted in ( a ) selection of two diluents which protected vaccinia virus against the effects of high relative humidity, ( b ) measurement of the effect of various chemical additives in the diluents, and ( c ) quantitative measurement of the moisture absorbed by the various dried products.