Premium
Growth of mouse fibroblast‐like cells in an autoclavable medium
Author(s) -
Orlando M. D.,
Bowersox O. C.,
Riley J. M.
Publication year - 1968
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260100105
Subject(s) - fibroblast , cell culture , suspension culture , population , biology , chemistry , microbiology and biotechnology , medicine , genetics , environmental health
A relatively simple, autoclavable medium was developed that would support the growth of the mouse fibroblast (MFL) cell line in suspension culture. This medium was prepared in three stages with decreasing quantities of serum. As the serum was reduced from 5% to none, the amount of Bacto‐Peptone was increased from none to 0.5%. The reduction and finally elimination of serum did not affect proliferation adversely, but actually seemed to produce more rapid growth and higher levels of cell population.