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Large‐scale isolation of prolyl‐ t RNA synthetase from Phaseolus aureus
Author(s) -
Dunnill P.,
Dunnill P. M.,
Houldsworth M.,
Boddy A.,
Lilly M. D.
Publication year - 1967
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260090307
Subject(s) - phaseolus , enzyme , ammonium sulfate precipitation , ammonium sulfate , rna , isolation (microbiology) , yield (engineering) , biochemistry , mung bean , glycine , trichloroacetic acid , ammonium , chemistry , enzyme assay , biology , chromatography , botany , food science , microbiology and biotechnology , amino acid , gene , organic chemistry , size exclusion chromatography , materials science , metallurgy
Some of the problems involved in the scale‐up of enzyme isolation procedures have been examined. In particular the isolation of prolyl‐ t RNA synthetase has been investigated. Using a modified procedure (500 × laboratory scale) the enzyme has been separated from 25 kg. of Phaseolus aureus (mung bean) and Partially purified by acid precipitation followed by fractional precipitation with ammonium sulfate. The yield of enzyme was 42 g. (10.5% recovery).