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Foam separation of bacteria with a cationic surfactant
Author(s) -
Grieves Robert B.,
Wang ShingLing
Publication year - 1967
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260090205
Subject(s) - serratia marcescens , bacillus cereus , proteus vulgaris , pulmonary surfactant , bacillus subtilis , pseudomonas fluorescens , chromatography , distilled water , bacteria , chemistry , filtration (mathematics) , escherichia coli , microbiology and biotechnology , biology , pseudomonas aeruginosa , biochemistry , genetics , statistics , mathematics , gene
An experimental investigation is presented of the foam separation of six species of bacteria: Escherichia coli, Serratia marcescens, Proteus vulgaris, Pseudomonas fluorescens, Bacillus cereus , and Bacillus subtilis var niger . A cationic surfactant, ethylhexadeeyldimethylammonium bromide is used and results are evaluated in terms of total cell count, using a membrane filtration technique. From similar neutral distilled water suspensions of the pure cultures (approximately 10 7 cells/ml.) and using the same operating conditions, ratios of cell concentrations in the residual suspensions to those in the initial suspensions range from 0.0013 for Bacillus subtilis var niger to 0.25 for Serratia marcescens. The presence of bacteria, compared to pure surfactant solutions, produces lower collapsed foam volumes; the foam volumes have a strong influence on the separations achieved with the various species, with enrichment ratios ranging from 27 to 3088 and residual ratios ranging from 0.001 to 0.247.