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Utilization of gas oil by a yeast culture
Author(s) -
Miller Thomas L.,
Johnson Marvin J.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080409
Subject(s) - wax , hydrocarbon , yeast , nitrogen , alkane , chemistry , pristane , substrate (aquarium) , candida tropicalis , chromatography , carbon source , biology , biochemistry , organic chemistry , ecology
A mixed yeast culture (Culture 4) was grown on representative gas oil samples as well as paraffin wax. Culture 4 was found to utilize n ‐paraffinic hydrocarbons almost quantitatively from most gas oil fractions; significant alteration of other hydrocarbon components was not detected. Generation times of 4.0–9.0hr. were typical during the exponential growth phase in fermentations with various gas oil fractions. Cell yields were 70–90% based on n ‐paraffin utilization. The culture appeared to exhibit maximum efficiency of n ‐alkane removal in the C 19 to C 24 range. The cells recovered from the fermentations contained 8.8–9.3% nitrogen. Paraffin wax also served as a suitable carbon source when dissolved in 2,6,10,14‐tertramethylpentadecane (pristane). However, substrate utilization appeared to be incomplete.