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Kinetics of polysaccharide B‐1459 fermentation
Author(s) -
Moraine R. A.,
Rogovin P.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080405
Subject(s) - polysaccharide , fermentation , chemistry , food science , xanthomonas campestris , yield (engineering) , nitrogen , limiting , biochemistry , organic chemistry , materials science , metallurgy , gene , mechanical engineering , engineering
Abstract Polysaccharide gum was made by fermentation with Xanthomonas campestris NRRL B‐1459 in a medium of glucose, minerals, and distillers' solubles. The effect of distillers' solubles on growth rate can be described by the familiar saturation equation. Although a quasistoichiometric relationship was observed between nitrogen utilization and growth, total nitrogen supply was not growth limiting, nor was polymer formation growth associated. Cell growth primarily took place in the early part of the fermentation; polysaccharide biosynthesis occurred throughout the fermentation. Glucose was converted to polysaccharide at a fairly constant yield, which was 70–80% of glucose consumed, under optimum conditions. The kinetic patterns observed indicate that multistage continuous fermentation will be suitable for polysaccharide production.