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Continuous‐culture system for ecological studies of microorganisms
Author(s) -
Parker R. B.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080403
Subject(s) - streptococcus salivarius , microorganism , peristaltic pump , microbiology and biotechnology , anaerobic exercise , biology , biological system , microbiological culture , biochemical engineering , ecology , chemistry , bacteria , streptococcus , physics , engineering , thermodynamics , physiology , genetics
An improved system is presented for measurement of interactions between a number of individual bacterial species. In the procedure, steady‐state populations are fed into a common‐mixed‐culture vessel. Generation times of each species are determined under these conditions and contrasted with generation time in an identical situation in pure culture. Populations for generation time are determined with the aid of differential media. The mechanical system includes three types of peristaltic pumps for media feed and a unit for measurement, recording, and/or control of pH. A new type of anaerobic continuous‐culture vessel which can be inoculated, sampled, and fed continuously is also described. A functional test of a three‐part system including Streptococcus salivarius , Veilloncella alcalescens , and Staphylococcus aureus is presented. An unusual feature was the finding that, under certain conditions, the generation time of S. Salivarius was less than 10 min.