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Sequential substrate removal by activated sludge after a change in salt concentration
Author(s) -
Kincan D. F.,
Gaudy A. F.,
Gaudy Elizabeth T.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080305
Subject(s) - lysis , salt (chemistry) , chemistry , substrate (aquarium) , activated sludge , biochemistry , fermentation , metabolism , chromatography , carbohydrate metabolism , chloramphenicol , food science , biology , wastewater , organic chemistry , waste management , antibiotics , ecology , engineering
Previous studies indicated that when cells grown in a NaCl‐free glucose medium were subjected to a high salt concentration, cellular constituents were released which were metabolized by the cells in preference to glucose. In the present study, cells grown on glucose in high salt medium were subjected to a shock loading of salt‐free medium. In this case, the resulting lysate was not used in preference to glucose; the lysate was metabolized only after an acclimation period following glucose utilization. It was shown by injecting chloramphenicol into the reaction liquor during glucose metabolism that new protein synthesis was required in order to metabolize the lysate. This response represents an additional way in which a rapid change in salt concentration can adversely affect biological treatment of waste waters, and a new type of situation in which sequential removal of substrates occurs.