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A chemically defined fermentation medium for the growth of Micromonospora purpurea
Author(s) -
Wagman G. H.,
Weinstein M. J.
Publication year - 1966
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260080207
Subject(s) - maltose , chemistry , sucrose , fermentation , zinc , nitrogen , mycelium , magnesium , potassium , food science , phosphorus , chemically defined medium , biochemistry , botany , biology , organic chemistry , in vitro
A chemically defined medium for Micromonospora purpurea has been devised, consisting of glucose, a nitrogen source, calcium carbonate, magnesium sulfate, dibasic potassium phosphate, and the required trace quantities of iron, copper, zinc, and manganese. Using washed cell inocula, dry mycelial weights of more than 16 mg./ml. were obtained in 7‐day shaken‐flask fermentations. Nutrient requirements for M. purpurea are discussed and growth data presented. Sucrose, maltose, starches and dextrins could be substituted for glucose, and resulted in good growth of the organism. A number of amino acids and inorganic nitrogen‐containing compounds were capable of utilization as sole nitrogen sources. Weekly serial transfers of the culture in defined medium have shown no diminution in mycelial weights over a four‐month period.